BiomeFUN 2025

15 - 19 September Belgrade, Serbia
From Genomic Analysis to Functional Models in Microbiomes and Synthetic Consortia
40
Spots
5
Days
14
Speakers

About BiomeFUN 2025

Microbial communities promise to revolutionize many fields, from biotechnology and health to agriculture. Mathematical and computational modeling is key to fulfill this potential. In this workshop, we will learn techniques to quantitatively analyze, understand and engineer microbial communities and synthetic microbial consortia. We will cover a range of topics, from the analysis of metagenomic sequencing data, to building and simulating meta-genome-scale metabolic models and analyzing the function of synthetic consortia using cutting-edge statistical "structure-function landscape" methods.

What you will learn

  • 16S and shotgun metagenomycs analysis
  • Functional genomics, profiling and visualization
  • Basic and advanced stoichiometric modeling with COBRA
  • Meta-genome scale modeling of microbial communities
  • Structure-function landscape modeling of microbial consortia
  • Introduction to uses of machine learning in the field

Event Starts In:

Grants available!

Thanks to our main sponsor, the Federation of European Microbiological Societies (FEMS), we are thrilled to offer up to 10 grants - covering registration fee and accommodation - aimed at participants from low and middle income countries. Please state in your registration if you would like to apply for one of the grants!

1. Selection Criteria for FEMS Supported Participants

  • Priority for FEMS-supported places will be given to PhD students and early-career postdoctoral researchers (within 5 years of PhD) working in microbiome analysis, SynCom communities and bioinformatics, or related fields.
  • Selection will be based on a motivation letter with relevance of the workshop to the applicant’s current research, abstract and CV. Geographic and gender diversity will also be considered.
  • Please note that the grants cover registration fee and accomodation. All participants are expected to cover their traveling expenses.

2. Criteria for selection of non-supported participants

  • Scientists with a Masters degree or above who are currently involved in Life Science research/Industry are encouraged to apply.

Organizers

Ivica Dimkić
Scientific & Organizing Committee Chairperson
Faculty of Biology - University of Belgrade, Serbia
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Djordje Bajić
Scientific & Organizing Committee Co-Chairperson
Delft University of Technology, the Netherlands
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Speakers and teachers

Marcel van den Broek
Bioinformatician
Delft University of Technology, the Netherlands
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Milan Dragićević
Senior AI Solution Developer
Parexel, Serbia
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Alfonso Esposito
Professor
University of Verona, Italy
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Alexandre Jousset
CEO of Cybiome GmbH
Cybiome GmbH, Switzerland
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João Pedro Saraiva
Senior Scientist
Helmholtz Centre for Environmental Research GmbH - UFZ, Germany
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Prateek Shetty
Postdoc
Matthias-Schleiden-Institut, Germany
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Timothy Páez-Watson
Postdoc
Delft University of Technology, the Netherlands
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Minke Gabriëls
PhD Candidate
Delft University of Technology, the Netherlands
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Karel Olavarria
Postdoc
Delft University of Technology, the Netherlands
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Juan Díaz-Colunga
Junior Group Leader
Spanish National Research Council, Spain
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Sascha Patz
Bioinformatician
Julius Kühn Institute (JKI) - Federal Research Center for Cultivated Plants, Germany
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Katarina Kruščić
PhD Candidate
Faculty of Biology - University of Belgrade, Serbia
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Uroš Gojković
PhD Candidate
Delft University of Technology, the Netherlands
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Schedule

Day 1: Fundamentals in Bioinformatics and Data Processing

Chairpersons: Marcel van den Broek & Milan Dragićević

8:00 - 9:00

Registration of participants

9:00 - 9:30

Welcome and Orientation

Speakers: Ivica Dimkić and Djordje Bajić
Introduction by organizers and workshop objectives

Speaker: Branko Jovčić
FEMS introduction

9:30 - 10:15

INVITED LECTURE: Introduction on Sequence Technologies, Common Bioinformatic File Formats and BASH for Genomics

Speaker: Marcel van den Broek
Overview of the next-generation sequencing technologies, essential bioinformatic file formats, and the role of Bash in building efficient workflows for large-scale data analysis.

10:15 - 10:30

Preparation break for the practical session

10:30 - 11:30

Practical 1.1 (part I): Intro Command Line, Cloud, Intro Sequencing, Quality Control, and Filtering

Speaker: Marcel van den Broek
Teaching assistants: Katarina Kruščić and Uroš Gojković
Basic programming skills tailored to microbiome data analysis, covering essential syntax and data manipulation. Practical exercises in sequence analysis and visualization.

11:30 - 12:00

Coffee break

12:00 - 13:00

Practical 1.1 (part II): Intro Command Line, Cloud, Intro Sequencing, Quality Control, and Filtering

Speaker: Marcel van den Broek
Teaching assistants: Katarina Kruščić and Uroš Gojković
Basic programming skills tailored to microbiome data analysis, covering essential syntax and data manipulation. Practical exercises in sequence analysis and visualization.

13:00 - 14:00

Lunch Break

14:00 - 17:00

Practical 1.2: Amplicon Data Analysis

Speakers: Milan Dragićević and Alfonso Esposito
Teaching assistants: Katarina Kruščić and Uroš Gojković
Hands-on training using R to import, preprocess, and filter sequencing data.

18:00 - 23:00

Poster Party with Catering and Drinks

Opportunity for participants to present their research and network

Day 2: Genome and Metagenome Analysis

Chairpersons: João Pedro Saraiva & Alfonso Esposito

9:00 - 09:45

INVITED LECTURE: Metagenomic Sequencing Approaches for Microbial Communities

Speaker: João Pedro Saraiva
Trace the evolution of metagenomics from marker-gene surveys to modern whole-genome shotgun, long-read, hybrid, and single-cell approaches, highlighting assembly, binning, annotation, and machine learning–based methods. Emphasizing their applications in ecology, health, and biotechnology.

9:45 - 10:00

Preparation break for the practical session

10:00 - 11:30

Practical 1.3 (part I): Metagenomics Assembly

Speakers: Alfonso Esposito and João Pedro Saraiva
Teaching assistants: Minke Gabriëls and Katarina Kruščić
Design and execute metagenomic workflows from raw sequencing data through assembly, binning, and functional annotation to statistical interpretation ensuring robust, reproducible insights into microbial community structure and function.

11:30 - 12:00

Coffee break

12:00 - 13:00

Practical 1.3 (part II): Metagenomics Assembly

Speakers: Alfonso Esposito and João Pedro Saraiva
Teaching assistants: Minke Gabriëls and Katarina Kruščić
Design and execute metagenomic workflows from raw sequencing data through assembly, binning, and functional annotation to statistical interpretation ensuring robust, reproducible insights into microbial community structure and function.

13:00 - 14:00

Lunch break

14:00 - 14:45

INVITED LECTURE: Functional Annotation of Metagenomes: From Contigs to Community Function

Speaker: Prateek Shetty
Overview of soil metagenome annotation, from gene prediction with Prokka to functional profiling with KEGG, COG, GO, and specialized databases such as CAZy and CARD, illustrated through agricultural case studies that highlight methodological choices, applications, and limitations.

14:45 - 15:00

Preparation break for the practical session

15:00 - 18:00

Practical 1.4: Functional Genomics and Data Visualization

Speaker: Prateek Shetty and Bogdan Jovanović
Teaching assistants: Minke Gabriëls and Katarina Kruščić
Running command-line annotation tools and performing R-based statistical analyses of functional abundance data, applying these workflows to soil-specific challenges in order to translate assembled contigs into meaningful ecosystem-level insights.

18:10 - 18:20

Group photo in front of the monument to Vojvoda Vuk on Topličin venac square

Day 3: Fundamentals of Modeling: From Theory to Data

Chairpersons: Alexandre Jousset & Djordje Bajić

9:00 - 10:00

KEYNOTE LECTURE: Scalable Digital Twin Framework for Microbiome Engineering and Functional Prediction

Speaker: Alexandre Jousset
Presenting a digital twin framework that uses metabarcoding data to predict microbiome responses, enabling rational design and functional manipulation across diverse environments.

10:00 - 10:45

INVITED LECTURE: Modelling Microbial Communities Across Scales to Understand Complexity and Guide Engineering

Speaker: Timothy Páez-Watson
Explore microbial community modelling across ecological, kinetic, and genome-scale levels, highlighting how different approaches can be integrated to understand complexity and guide the engineering of microbial communities.

10:45 - 11:00

Preparation break for the practical session

11:00 - 11:45

Practical 2.1 (part I): Introduction to Theoretical Models in Microbial Ecology and Python Practice

Speakers: Djordje Bajić and Timothy Páez-Watson
Teaching assistants: Uroš Gojković and Minke Gabriëls
Introduce theoretical models in microbial ecology, covering ecological, kinetic, and genome-scale approaches, with practical Python exercises to apply these models for analyzing and predicting community dynamics.

11:45 - 12:00

Coffee break

12:00 - 13:00

Practical 2.1 (part II): Introduction to Theoretical Models in Microbial Ecology and Python Practice

Speakers: Djordje Bajić and Timothy Páez-Watson
Teaching assistants: Uroš Gojković and Minke Gabriëls
Introduce theoretical models in microbial ecology, covering ecological, kinetic, and genome-scale approaches, with practical Python exercises to apply these models for analyzing and predicting community dynamics.

13:00 - 14:00

Lunch break

14:00 - 14:45

INVITED LECTURE: From Kinetics to Stoichiometric Models in Metabolic Networks

Speaker: Karel Olavarria
Explore how enzyme kinetics and metabolic fluxes can be translated into stoichiometric models to simulate and analyze microbial metabolic networks

14:45 - 15:00

Preparation break for the practical session

15:00 - 18:00

Practical 2.2: Practical Introduction to Metabolic Network Modeling

Speaker: Timothy Páez-Watson
Teaching assistants: Minke Gabriëls and Uroš Gojković
Introduction to metabolic network modeling, covering resource allocation constraints and dynamic flux balance analysis to simulate and analyze multi-species microbial communities.

Day 4: Genome and Metagenome-Scale Metabolic Modeling

Chairpersons: Karel Olavarria & Prateek Shetty

9:00 - 9:45

INVITED LECTURE: Thermodynamic and Kinetic Analysis of Metabolic Networks

Speaker: Karel Olavarria
Review of the key kinetic and thermodynamic principles that govern enzyme activity, metabolic fluxes, substrate consumption, and microbial growth, highlighting how these laws connect molecular reactions to population-level dynamics

9:45 - 10:00

Preparation break for the practical session

10:00 - 11:30

Practical 2.3: Thermodynamic Analysis of Metabolic Networks

Speaker: Karel Olavarria
Teaching assistants: Minke Gabriëls and Djordje Bajić
The kinetic principles and thermodynamic constraints governing enzyme-catalyzed reactions and metabolic fluxes, showing how they can be applied to analyze and model microbial metabolic networks

11:30 - 12:00

Coffee Break

12:00 - 13:00

INVITED LECTURE: Resource Allocation and Dynamic Metabolic Modeling of Microbial Communities

Speaker: Djordje Bajić
Explore resource allocation principles and dynamic metabolic modeling using stoichiometric and flux balance approaches to simulate and analyze multi-species microbial communities

13:00 - 14:00

Lunch Break

14:00 - 14:45

Practical 2.4 (part I): Resource Allocation and Dynamic Metabolic Modeling of Microbial Communities

Speaker: Djordje Bajić
Teaching assistants: Minke Gabriëls and Uroš Gojković
Covering global resource allocation principles and dynamic metabolic modeling using stoichiometric and flux balance approaches to simulate and predict the behavior of multi-species microbial communities

14:45 - 15:00

Preparation break for the practical session

15:00 - 18:00

Practical 2.4 (part II): Resource Allocation and Dynamic Metabolic Modeling of Microbial Communities

Speaker: Djordje Bajić
Teaching assistants: Minke Gabriëls and Uroš Gojković
Covering global resource allocation principles and dynamic metabolic modeling using stoichiometric and flux balance approaches to simulate and predict the behavior of multi-species microbial communities

Day 5: Statistical Approaches and AI in Microbial Ecology

Chairpersons: Juan Díaz-Colunga & Sascha Patz

9:00 - 9:45

INVITED LECTURE: Structure-Function Mapping for Microbial Consortia

Speaker: Juan Díaz-Colunga
Explore how microbial communities exhibit complex interspecies interactions, yet often display surprisingly predictable functions that can be captured with simple statistical models analogous to global epistasis in genetics, providing a unifying framework to link structure and function across biological scales and paving the way for rational community design

9:45 - 10:00

Preparation break for the practical session

10:00 - 11:30

Practical 3.1 (part I): Structure-Function Landscape Analysis and Prediction

Speaker: Juan Díaz-Colunga
Teaching assistants: Uroš Gojković and Djordje Bajić
Applying statistical modeling approaches to microbial community data, learning how to identify predictable functional patterns from complex interaction networks and using this knowledge to begin designing communities optimized for biotechnological applications

11:30 - 12:00

Coffee break

12:00 - 13:00

Practical 3.1 (part II): Structure-Function Landscape Analysis and Prediction

Speaker: Juan Díaz-Colunga
Teaching assistants: Uroš Gojković and Djordje Bajić
Applying statistical modeling approaches to microbial community data, learning how to identify predictable functional patterns from complex interaction networks and using this knowledge to begin designing communities optimized for biotechnological applications

13:00 - 14:00

Lunch Break

14:00 - 15:00

INVITED LECTURE: Introduction to Machine Learning in Microbiome Science

Speaker: Sascha Patz
Introducing machine learning and deep learning methods to microbiome research, highlighting their applications from community profiling to predictive modeling in health and agriculture, while addressing challenges of data complexity, bias, and interpretability to ensure reliable and biologically meaningful insights

15:15 - 15:45

Closing Discussion

Speakers: Course Board & Participants
Reflection on workshop outcomes, future directions in microbiome research, and opportunities for collaboration

16:00 - 18:00

Free Walking Tour: Kalemegdan Park and Belgrade Fortress

19:00 - 00:00

Unforgettable Gala Dinner

Join us for live music, delicious food, Serbian drinks, engaging conversations, and a celebration of outstanding workshop achievements!

Register

Early Bird Registration

Regular/Professionals: €350
Students and Early Career Scientists: €250

For payments before 30 June 2025

Regular Registration

Regular/Professionals: €450
Students and Early Career Scientists: €350

From 1 July to 1 August 2025

On-site Registration


Regular/Professionals: €550
Students and Early Career Scientists: €450

Note: All payments made after August 1st will be charged at the same rate as on-site registration

Important to consider when applying for a fellowship:

Abstract, CV and motivation letter are required for fellowship applicants. Please submit these in the corresponding section in the registration form.

Abstract submission deadline for professionals: May 25 June 15

Abstract and grant status confirmation: May 30 June 20

Download abstract template

What's included?

  • 9 talks from leading experts in bioinformatics
  • Access to 9 workshops
  • Amazing after-parties
  • Coffee & Snacks breaks, Lunches, Workshop dinner
  • Certificate of attendance on course completion
  • Materials and comprehensive resources, including access
    to relevant datasets and online tools for extended learning

Venue

Hotel Palace

Adress: Topličin Venac 23, 11000 Belgrade

About Hotel

Palace Hotel Belgrade is located in the very heart of Belgrade, in the immediate vicinity of the main pedestrian area of Knez Mihailova Street. It has a very convenient location in a quiet part of the city, close to historical and cultural sights, city and state institutions, museums, theaters, cinemas, numerous shopping centers as well as Shkadarlija - the old bohemian quarter. It is intended for vacation both for business people and for those who travel for pleasure. Palace Hotel Belgrade is categorized as a 4-star hotel.

How To Get Here

By Bus

1. By Bus from Nikola Tesla Airport:
Take bus 72 from the airport and exit at the 'Brankov Most' station (about a 30-35 minute ride). From there, it's a 310-meter walk to the Palace Hotel.

2. By Minibus from Nikola Tesla Airport:
Alternatively, you can take minibus A1 from the airport and exit at the last stop, Slavija. From here, you have two options:

  • Take a 15-minute walk through the city center to reach the hotel.
  • Or, catch one of the following public transport options:
    • Bus 31, Eco2, or trolley 29, and exit at 'Studentski Trg' station. The hotel is just a 2-minute walk from the station.
Note: Public transportation in Belgrade is free.

By Taxi

Alternatively, you can take a taxi from Nikola Tesla Airport. Please note that the ride should cost around €30. It’s recommended to discuss the price with the driver before you get in.

Contact

For any further information, feel free to contact us via our email!

Agency contact



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For information about payment and invoice, please contact our agency "Impala"

+381-11-249-78-82

office@impalayu.com

Adress



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Toplicin Venac 23
11000 Belgrade